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KMID : 0648620050100020048
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Korean Journal of Nosocomial Infection Control
2005 Volume.10 No. 2 p.48 ~ p.56
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Prevalence and Molecular Genetic Characteristics of Vibrio parahaemolyticus New O3:K6 and O4:K68
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Choi Tae-Yeal
Pai Hyun-Joo
Choi Won-Ho
Kim Duk-Un
Kang Jung-Oak
Ahn You-Hern
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Abstract
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BACKGROUND: Vibrio parahaemolyticus belonged to the new 03:K6 serotype was demonstrated an unusual potential to spread and an enhanced propensity to cause infection in the worldwide. Recently, increased numbers of V. parahaemolyticus 03:K6 had been isolated in Korea, and we analyzed the prevalence and molecular genetic characteristics of V. parahaemolyticus 03:K6 isolates.
METHODS: V. parahaemolyticus were isolated from clinical specimens of patients with diarrhea in Hanyang University Hospital, Seoul, Korea, from 1998 to 2005. The serovars of isolates were determined by slide agglutination test with specific antisera. All isolates were examined for the presence of tdh/trh genes. AGS-PCR method detecting the new 03:K6 clone was used in this study. We analyzed clonality of these isolates by infrequent restriction site-polymerase chain reaction (IRS-PCR).
RESULTS: Thirty-six strains were isolated from 1998 to 2005. The main serotype of isolates were 03:K6 (24/36: 67%), All of them have tdh gene but not trh gene and an unique toxRS gene of the new 03:K6. The morphotypes of 03:K6 isolates show a same pattern of IRS-PCR, but can easily be differentiated from non-03:K6 and 04:K68 isolates.
CONCLUSION: The 03:K6 was a main serotype of V. parahaemolyticus isolated from 1998 to 2005 and they show same molecular characteristics.
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KEYWORD
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Vibro parahaemoyticus, Serotypes, tdh gene, trh gene, Genotyping
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